Evaluation of PGE2 synthesis by Electron Paramagnetic Resonance

PGE2 and other prostanoids are measured using specific KITs and the activity of the enzymes involved in their formation is assessed indirectly. In this context, we propose the use of a tool for the direct evaluation of enzymes, since they act robustly in the escape mechanisms of neoplastic cells. Electron Paramagnetic Resonance (EPR) was chosen because it measures the release of free radicals and has provided surprising results. We therefore hope that the technique can also be used by researchers in the biological and health sciences.