Basic Protocols in Foods and Nutrition

Part I: Animal Experimentation
1. Experimental Diets (Normocaloric and Hypercaloric Diets)
Laís Marinho Aguiar, Juliana Kelly da Silva Maia, Cinthia Baú Betim Cazarin

2. Measures of Food Intake, Body Weight Gain, and Energy Efficiency in Mice - Cíntia Reis Ballard and Cinthia Baú Betim Cazarin

3. The assessment of glucose homeostasis in rodents: Glucose, Insulin and Pyruvate Tolerance Tests Nathalia R. V. Dragano and Edward Milbank

4. Histopathological Evaluation of Steatohepatitis in Animal Experiments
Yoshihisa Takahashi, Erdenetsogt Dungubat, Toshio Fukusato

5. Quantification of short-chain fatty acids in feces
Mirella Romanelli Vicente Bertolo, and Stanislau Bogusz Junior

6. Quantification of non-esterified fatty acids in serum and plasma
Mirella Romanelli Vicente Bertolo, and Stanislau Bogusz Junior

7. Lipid Peroxidation (TBARS) in biological samples
Lilian R. B. Mariutti

8. Extraction of Bile Acids from Biological Samples and Quantification Using Ultra-High-Performance Liquid Chromatography-Orbitrap Mass Spectrometry
Shota Hori, Hongxia Liu, Riho Yamada, Shun Ichitsubo, Ayana Sakaguchi, Fumika Yokoyama, and Satoshi Ishizuka

9. Glycogen measurement
Cavaglieri, C.R.; Silva, C.A.; and Bernardes, C.F.

10. Evaluation of the minerals and trace elements in the biological samples
Duygu Aydemir and Nuriye Nuray Ulusu

11. Microbiome Evaluation
Alba Rodríguez-Nogales, Antonio Jesús Ruiz-Malagón, Jose Alberto Molina-Tijeras, Maria Elena Rodríguez-Cabezas, Julio Gálvez

12. The GSH colorimetric method as measurement of antioxidant status in serum and rodent tissues
Milena Morandi Vuolo, Juliana Kelly Silva Maia, Ângela Giovana Batista

13. Guidance for designing a preclinical bioavailability study of bioactive compounds
Helena Dias de Freitas Queiroz Barros, Cinthia Ba├║ Betim Cazarin, Mario Roberto Mar├│stica Junior


Part II: Clinical Trials
14. Protocols related to Nutritional Anamnesis in Head and Neck Cancer Patients
Judith B├╝ntzel and Jens B├╝ntzel

15. Food Diary, Food Frequency Questionnaire, and 24-hour dietary recall
Luisa Saravia, Paula Moliterno, Estela Skapino, and Luis A. Moreno

16. The Use of Questionnaires to Measure Appetite
James H Hollis

17. Protocols for the Use of Indirect Calorimetry in Clinical Research
Katherine L Ford, Camila LP Oliveira, Stephanie Ramage, and Carla M Prado

18. Anthropometric Assessment Methods for Adults and Older People
Thalita Cremonesi Pereira and Cinthia Ba├║ Betim Cazarin

19. MRI-based Body Composition Analysis
Magnus Borga, André Ahlgren, and Sarah Weston

20. Functional tests for assessing human beta-cell function and insulin sensitivity
Marcelo Miranda de Oliveira Lima and Bruno Geloneze

21. Maximum oxygen consumption - VO2max laboratory assessment
Mara Patrícia Traina Chacon-Mikahil, Alex Castro, Danilo dos Santos Caruso, and Arthur Fernandes Gáspari

2. 2Muscle and Fat Biopsy and Metabolomics
Cláudia Regina Cavaglieri, Mara Patrícia Traina Chacon-Mikahil., Renata Garbellini Duft, Ivan Luiz Padilha Bonfante, Arthur Fernandes Gáspari, and Alex Castro