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This volume explores techniques that study interactions between proteins in different species, and combines them with context-specific data, analysis of omics datasets, and assembles individual interactions into higher-order semantic units, i.e., protein complexes and functional modules. The chapters in this book cover computational methods that solve diverse tasks such as the prediction of functional protein-protein interactions; the alignment-based comparison of interaction networks by SANA; using the RaptorX-ComplexContact webserver to predict inter-protein residue-residue contacts; the docking of alternative confirmations of proteins participating in binary interactions and the visually-guided selection of a docking model using COZOID; the detection of novel functional units by KeyPathwayMiner and how PathClass can use such de novo pathways to classify breast cancer subtypes. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary hardware- and software, step-by-step, readily reproducible computational protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Protein-Protein Interaction Networks: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this evolving field.

This third edition volume expands on the previous editions with updated approaches and techniques used to study protein nanotechnology and the future of nanomaterial compositions. This book is organized into Three Parts: Part One looks at recombinant protein expression in insect cells, and methods to produce molecular motors, molecular superglues, and protein templates; Part Two explores functionalization strategies and ways to incorporate functional protein components into nanodevices; Part Three discusses various instrumental techniques used to study protein nanostructures. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Protein Nanotechnology: Protocols, Instrumentation,and Applications, Third Edition is a valuable resource for any researchers looking to expand their knowledge in this evolving field.

This book provides a toolkit of novel research approaches for investigators to study diabetic nephropathy, including critical experimental models from the fly to the fish, cells in culture, and in vivo mammalian approaches. The collection also explores powerful techniques to image the kidney, such as traditional histological techniques as well as electron, confocal, and two-photon microscopy, pathophysiology of the diabetic kidney, and gene editing and regenerative medicine. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Diabetic Nephropathy: Methods and Protocols seeks to foster new research directions and inspire ideas to enhance our understanding of diabetic nephropathy and todevelop treatments for this condition.

This detailed book contains techniques to explore the unusual properties of the peculiar microsatellites known as trinucleotide repeats. Beginning with technical challenges raised by secondary structure-forming repeats and their propensity to contract and expand over time, the book continues with genetic screens in Saccharomyces cerevisiae, experimental systems to study trinucleotide repeat instability in human cells, dedicated experimental systems in appropriate cell types, as well as gene therapy approaches using the CRISPR-Cas family of endonucleases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Trinucleotide Repeats: Methods and Protocols serves as avaluable aid to experts and newcomers alike who seek to investigate this fascinating and ever-expanding field of study.

This book provides a thorough introduction to widely used techniques for the study of the intersection between developmental biology and neuroscience, an exceptional area to address and investigate impacting biological questions. The fully updated volume examines cutting-edge techniques on a representative range of animals, including widely used genetic model systems, such as the fruit fly, zebra fish, chicken, and mouse, as well as non-canonical experimental systems opened up through the advent of genome editing. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Brain Development: Methods and Protocols, Second Edition is an ideal guide for researchers interested in utilizingrecent technical advances in molecular genetics for the study of the brain.