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The accurate and reliable diagnosis of transmissible diseases is the most powerful weapon available to ensure their control, and in some cases eradication. Indeed many viral infections display clinical signs that are indistinguishable from bacterial or parasitic infections.

A panel of internationally recognized research scientists and clinical investigators brings together a diverse collection of readily reproducible methods for identifying and quantifying a large number of specific genetic abnormalities associated with the broad spectrum of myeloid malignancies. Highlights include techniques for the detection of BCR-ABL mutations and resistance to imatinib mesylate, detection of the FIP1L1-PDGFRA fusion in idiopathic hypereosinophilic syndrome and chronic eosinophilic leukemia, classification of AML by DNA-oligonucleotide microarrays, and detection of the V617F JAK2 mutation in myeloproliferative disorders. In addition to gene rearrangments, other prognostically relevant molecular lesions such as FLT3 mutations and WT-1 overexpression are covered.

In particular, the methods can be used to identify in clinical samples those genetic mutations responsible for such congenital abnormalities as Marfan syndrome, Williams-Beuren Syndrome, Alagille syndrome, Noonan syndrome, and Friedreich ataxia.

Comprehensive and up-to-date, the two volumes of Cartilage and Osteoarthritis provide researchers and bench scientists with readily reproducible protocols for new experiments to understand-from the cellular to the animal level-the pathophysiology of cartilage and to discover molecular targets for pharmacological intervention.

This work, Lung Cancer, Volume 2: Diagnostic and Therapeutic Methods and Reviews, in the Methods in Molecular Medicine series presents an overview of the current status of those methods useful in the diagnosis and tre- ment of lung cancer-both as it exists in the clinic and as it is being revoluti- ized in the laboratory.

The identification of hepatitis C virus by Michael Houghton and his c- leagues at the Chiron Corporation nearly years ago represented a technical tour de force of modern molecular medicine.

From the viewpoint of this volume, lung inflammation is now recognized as central to the pathophysiology of a number of severe respiratory conditions, the two most common being asthma and chronic obstructive pulmonary disease (COPD).

Laboratory studies in hemostasis have traditionally focused on abn- malities of platelet function or the quantitative and qualitative disorders that affect the proteins involved in blood coagulation.

Since the first recognition of outbreaks of cerebrospinal or spotted fever at the end of the nineteenth and the beginning of the twentieth centuries, the menace of the meningococcus has been high on the list of public health prio- ties.

In Drug Targeting: Strategies, Principles, and Applications we have tried to compile a state-of-the-art volume on current targeting approaches.

Harry Baker and Rosalind Ridley have done an admirable job in assem bling this collection of articles that describe the methodology frequently used to study a group of CNS illnesses often referred to as the "prion diseases."

In Chapter 1, Dokal and colleagues present simple protocols for the molecular investigation of Fanconi anemia and dyskeratosis congenita. In Chapter 3, Old details protocols for the molecular diagnosis of most forms of hemoglobinopathy.

The Methods in Molecular Medicine series is intended as a resource for both novice and experienced investigators attempting to diversify their tech- logical base in research.

Psychiatric Genetics provides the reader with a complete view of the methodological problems encountered in psychiatry genetics and proposes solutions to commonly occurring questions.

Internationally recognized investigators review the latest developments in, and novel approaches to, understanding the prion protein and prion diseases at the molecular level.

In keeping with the organization used effectively in other volumes in this series, each chapter has a brief introduction followed by a list of required items, protocols, and notes designed to help the reader perform the expe- ments without difficulty.

A comprehensive manual in the broad and deep field of cardiovascular medicine, this volume covers a wide spectrum of techniques encompassing biochemical, pharmacological and molecular biology disciplines currently used to assess vascular disease progression.

Cardiovascular disease is the leading cause of death in developed countries, but is quickly becoming an epidemic in such well-populated countries as China, India, and other developing nations.

Cardiovascular disease is the leading cause of death in developed countries, but is quickly becoming an epidemic in such well-populated countries as China, India, and other developing nations.

The aim of Hematologic Malignancies: Methods and Techniques is to review those methods most useful for the diagnosis and subsequent mana- ment of hematologic malignancies.

We are in an exciting era in the war against cancer, with real prospects for novel anticancer drugs that are cancer cell-specific without the toxicities that have been the hallmark of conventional cytotoxic cancer chemotherapy.

This work, Lung Cancer, Volume 2: Diagnostic and Therapeutic Methods and Reviews, in the Methods in Molecular Medicine series presents an overview of the current status of those methods useful in the diagnosis and tre- ment of lung cancer-both as it exists in the clinic and as it is being revoluti- ized in the laboratory.

The unexpected and unp- dictable occurrence of the disease in previously healthy children and young adults, its rapid progression, and the frequent occurrence of purpura fulminans with the resulting gangrene of limbs and digits and the requirement for mutilating s- gery, have all heightened both public and medical interest in the disease.

Pancreatic Cancer: Methods and Protocols provides a broad range of protocols for molecular, cellular, pathological, and statistical analyses of s- radic and familial pancreatic cancer.

Cardiovascular disease is the leading cause of death in developed countries, but is quickly becoming an epidemic in such well-populated countries as China, India, and other developing nations.

Cardiovascular disease is the leading cause of death in developed countries, but is quickly becoming an epidemic in such well-populated countries as China, India, and other developing nations.

The last decade has witnessed a steady stream of new synthetic approaches to peptide mimetics, compounds that replace phy- ologically vulnerable peptide functionalities with chemical modules of - creased stability and cellular penetration.

A timely primer on the new reality of antisense and RNA inhibition for treating a broad range of diseases. The authors show how antisense oligonucleotides are being designed and studied in relation to hypertension, various cancers, inflammatory bowel disease, brain disorders, the blood-brain barrier, and drug delivery.

Opioid research is one of the multidisciplinary research areas that involve advanced techniques ranging from molecular genetics to neuropharmacology, and from behavioral neuroscience to clinical medicine.