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Hemoglobin has been involved in the most significant advances in our understanding of modern genetics and molecular biology. Part of the "Methods in Enzymology" series, this volume covers: preparation, purification, and analysis of human hemoglobin and of various non-human hemoglobins; preparation of chemically modified hemoglobins; and more.

Details methods for the study of RNA processing which pertain to preparation of substrates and processing enzymes or factors, and to analysis of the resulting products. Includes methods for the study of the structures of precursors, cofactors, and enzymes or complexes that catalyze function at indi

Talks about the methods for the assay, purification, and characterization of adenylyl cyclases, guanine nucleotide-dependent regulatory proteins (G proteins), and guanylyl cyclases. This book looks at the preparation of materials useful in purification of components of hormonally responsive cyclase systems; purification; regulation; and more.

Useful for researchers in various fields of life sciences, this title includes features such as cell cycle regulators in mammalian systems, cell cycle control in yeast and fungal systems, and analysis of cell cycle regulators in oocyte, egg, and embryonic systems, as well as general methods.

Contains articles that reflect the integration of molecular genetics with photosynthesis. This work focuses on the genetic engineering of the photosynthesis apparatus, mechanisms for genetic selection of photosynthetic mutants, biophysical and biochemical analysis of Photosystem I and Photosystem II Mutations, and Photosynthetic Gene Expression.

Synthetic Biology and Metabolic Engineering in Plants and Microbes: Part A, the new volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods, synthetic biology, and metabolic engineering in plants and microbes, and includes sections on such topics as the uses of integrases in microbial engineering, biosynthesis, and engineering of tryptophan derived metabolites, regulation and discovery of fungal natural products, and elucidation and localization of plant pathways.

Computational Approaches for Studying Enzyme Mechanism Part A, is the first ofá two volumes in the Methods in Enzymology series, focusses on computational approaches for studying enzyme mechanism. The serial achieves the critically acclaimed gold standard of laboratory practices and remains one of the most highly respected publications in the molecular biosciences. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 550 volumes, the series remains a prominent and essential publication for researchers in all fields of life sciences and biotechnology, including biochemistry, chemical biology, microbiology, synthetic biology, cancer research, and genetics to name a few.Focusses on computational approaches for studying enzyme mechanismContinues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in intermediate filament associated proteins, and contains sections on such topics as lamin-associated proteins, intermediate filament-associated proteins and plakin, and other cytoskeletal cross-linkers

Synthetic Biology and Metabolic Engineering in Plants and Microbes, Part B, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods, synthetic biology, and metabolic engineering in plants and microbes, and includes sections on such topics as the usage of integrases in microbial engineering, biosynthesis, and engineering of tryptophan derived metabolites, regulation and discovery of fungal natural products, and elucidation and localization of plant pathways.Continues the legacy of this premier serial with quality chapters authored by leaders in the field of enzymologyContains two volumes covering research methods in synthetic biology and metabolic engineering in plants and microbesIncludes sections on such topics as the uses of integrases in microbial engineering, biosynthesis and engineering of tryptophan derived metabolites, regulation and discovery of fungal natural products, and elucidation and localization of plant pathways

De Novo Enzyme Design, the newest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume includes the design of metal binding maquettes, insertion of non-natural cofactors, Cu metallopeptides, non-covalent interactions in peptide assemblies, peptide binding and bundling, heteronuclear metalloenzymes, florinated peptides, De Novo imaging agents, and protein-protein interaction. Continues the legacy of this premier serial with quality chapters on de novo enzyme designRepresents the newest volume in the Methods in Enzymology series, providing premier, quality chapters authored by leaders in the field Ideal reference for those interested in the study of enzyme design that looks at both structure and mechanism

cryoEM, a new volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods and new developments in recording images, the creation, evaluation and validation of 3D maps from the images, model building into maps and refinement of the resulting atomic structures, and applications of essentially single particle methods to helical structures and to sub-tomogram averaging.Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods that determine the structures of biological molecules, a vital step for understanding their functionContains the technical developments underpinning the advances of cryoEM and captures the exciting insights that have resulted

Single-Molecule Enzymology, Part B, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion.Continues the legacy of this premier serial with quality chapters authored by leaders in the fieldCovers research methods in single-molecule enzymologyContains sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion

Enzymology at the Membrane Interface, the latest volume in the Methods in Enzymology series, covers a subset of enzymes that work in the environment of the biological cell membrane. This field, called interfacial enzymology, involves a special series of experimental approaches for the isolation and study of these enzymes.Covers a subset of enzymes that work in the environment of the biological cell membraneOffers a series of experimental approaches for the isolation and study of enzymes