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This volume and its companion, Volume 214 of "Methods of Enzymology" seek to present a comprehensive, state-of-the-art compilation of the molecular and cellular methodology needed for pursuing research with carotenoids.

Talks about fluorescence in biophysics; fluorescence as a tool for studying proteins; determination of ground-state dissociation constant by fluorescence spectroscopy; fluorescence spectroscopy of protein-protein and protein-nucleic acid interactions; fluorescence methods for studying equilibrium macromolecule-liquid interactions; and more.

Part of a series which contains material useful for researchers in various fields of life sciences. This title features spectroscopic detection of reaction intermediates; isotopic and kinetic detection of reaction intermediates; and, chemical trapping and inhibitor methods for detecting reaction intermediates.

The aim of this volume is to give recombinant DNA researchers current knowledge about heterologous gene expression in four commonly employed organisms: escherichia coli, bacillus subtilis, yeast and mammalian cells.

To understand the structures and dynamics of DNA and its interactions with ligans, complete analysis of its confirmational flexibility is required. This volume and Volume 212 of "Methods in Enzymology" collate in one source the methods and approaches needed to achieve this goal.

Oncogenes have become a central focus of cancer research. This book helps those involved in this discipline of biochemistry in developing protocols for their research. It covers topics such as cell culture, molecular cloning, Gene function, protein-protein interactions, and protein-DNA interactions.

Small GTPases play a role in aspects of contemporary cell biology such as: control of cell growth and differentiation; regulation of cell adhesion and cell movement; and more. This volume covers various biochemical and biological assays in use for analyzing the role of small GTPases in many aspects of cell biology at the molecular level.

This volume and its companion, Volume 284, consolidate methods for purifying, characterizing, isolating, and applying lipases, and cover their uses in drug design and as additives in detergent development.

Provides: a collation of the methods for the identification, preparation, and quantification of vitamins and coenzymes; details on physical, chemical, and biological properties of vitamins and coenzymes; chemical and biological syntheses of vitamins, coenzymes, and their analogs; and aspects of transport and metabolism of vitamins and coenzymes.

Part of the ""Methods in Enzymology" series, this volume describes the methods for detection and characterization of redox-active prosthetic groups, which arise from pre-existing amino acid side chains, in a range of enzyme systems.

This volume, and its companions - "Methods of Enzymology" volumes 330 and 334 - cover contemporary knowledge concerning hyperthermophilic enzymes. Major topics in this volume include the enzymes of central metabolism, respiratory enzymes and biophysical and biochemical aspects of protein stability.

Presents spectroscopic and physical methods for the determination of metal ion environments in metalloenzymes and metalloproteins. Major topics include spectroscopic methods for the determination of metal ion environments in metalloenzymes and metalloproteins.

Presents methods related to the role of anaerobic dissimilatory cycles and sulfur metabolism. This volume deals with topics such as: sulfate transport, dissimilatory sulfate metabolism, dehydrogenases, hydrogenases from sulfate-reducing bacteria, electron carrier protein sulfate-reducing bacteria, and dissimilatory sulfur reduction.

Provides information on prevention of oxidative stress; redox regulation of metabolism; toxicology; and, carcinogenesis.

This volume of Methods in Enzymology is a companion to Volume 347 and addresses direct sensing of reactive oxygen species and related free radicals by thiol enzymes and proteins.

Part of a series which contains material useful for researchers in various fields of life sciences. This title features protein structure and function and nucleic acids and genes.

Principal techniques used in membrane fusion research are presented in this volume. Their shortcomings are evaluated and artifacts that may arise are discussed.

The Cumulative Subject Index for Volumes 195-198 and 200-227 includes the contents of the volumes covered, the subject index, contributor index, and a complete list of all the volumes published in the series. This volume supplements the Methods in Enzymology Cumulative Index Volumes 33, 75, 120, 140, 175, and 199.

This volume of METHODS IN ENZYMOLOGY and its companion Volume 233 present methods developed to investigate the roles of oxygen radicals and antioxidants in disease.

Provides: a collation of the methods for the identification, preparation, and quantification of vitamins and coenzymes; details on physical, chemical, and biological properties of vitamins and coenzymes; chemical and biological syntheses of vitamins, coenzymes, and their analogs; and aspects of transport and metabolism of vitamins and coenzymes.

This volume aims to provide the reader with an introduction to data analysis in biochemistry, using examples from biochemical research. It is designed for use by those with little background in mathematics.

Chemokines are major mediators of immune cells and are involved in a range of proinflammatory human diseases, including rheumatoid arthritis, multiple sclerosis, and organ transplant rejection. This book presents experimental protocols used in this field of research and includes topics such as C-X-C Chemokines, C Chemokines, and others.