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Enzymes in RNA Science and Biotechnology, Volume 691 in the Methods in Enzymology series, highlights new advances in the field, including chapters on Reverse transcriptase Part I (discovery, preparation, general utilization, MarathonRT for routine RT-PCR and for cDNA synthesis on challenging RNA templates Structured RNAs, repeat RNAs and more, Engineering TNA Polymerases Through Iterative Cycles of Directed Evolution, Reverse transcriptase Part II (RNA structure mapping and determination), RNA G-quadruplex (rG4) structure detection using RTS and SHALiPE assays, tRNA Structure-seq in vivo and in droplets, Capture the in vivo intact RNA structurome by CAP-STRUCTURE-seq, and more.

Presents methods and protocols dealing with thiol oxidation-reduction reactions and their implications as they relate to cell signaling. This title gathers various techniques from global labs, offering different methods.

Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Prokaryotic and Eukaryotic Systems, Volume 638, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Sample chapters from this new release include In vitro characterization of the colibactin-activating peptidase ClbP enables development of a fluorogenic activity probe, Using FDAA probes to study cell division in Bacillus subtilis, Chemoenzymatic synthesis of UDP-sugars, Chemical tools for selective activity profiling of bacterial penicillin-binding proteins, Chemical Probes Reveal and Extraseptal Mode of Cross-linking in Staphylococcus Aureus, and much more. Provides the authority and expertise of leading contributors from an international board of authorsPresents the latest release in the Methods in Enzymology seriesIncludes the latest information on retinoid signaling pathways

Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Optical Imaging and Conjugation, Volume 639, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Fluorogenic detection of protein aggregates in live cells using the AggTag method, Synthesis and Application of Ratiometric Probes for Hydrogen Peroxide Detection, Chemical Tools for Multicolor Protein FRET with Tryptophan, Fluorescing Isofunctional Ribonucleosides for Adenosine Deaminase Activity and Inhibition, Temporal profiling establishes a dynamic S-palmitoylation cycle, Solvation-guided design of fluorescent probes for discrimination of amyloids, and much more. Provides the authority and expertise of leading contributors from an international board of authorsPresents the latest release in the Methods in Enzymology seriesIncludes the latest information on retinoid signaling pathways

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. Provides the authority and expertise of leading contributors from an international board of authorsPresents the latest release in the Methods in Enzymology series

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field.Provides the authority and expertise of leading contributors from an international board of authorsPresents the latest release in the Methods in Enzymology series

The ability of polypeptides to form alternatively folded, polymeric structures is recognized as a major new frontier in protein research. This volume contains protocols and methodological insights into the methods for investigating the structures, mechanisms of formation, and biological activities of this important class of protein assemblies.

This volume and its companion, Volume 338 , supplement Volumes 176, 177, 239 , and 261 . Chapters are written with a "hands-on" perspective. That is, practical applications with critical evaluations of methodologies and experimental considerations needed to design, execute, and interpret NMR experi

This second volume on ribonucleases provides up-to-date, methods-related information on these enzymes. Of particular interest to researchers will be the discussion of artificial and engineered ribonucleases, as well as the application of ribonucleases in medicine and biotechnology.The critically ac

Suitable for researchers in various fields of life sciences, this book presents methods related to the use of bacterial genetics for genomic engineering. It includes sections on strain collections and genetic nomenclature; transposons; and, phage.

Produced by microbes on a large scale, methane is an important alternative fuel as well as a potent greenhouse gas. This title focuses on microbial methane metabolism, which is central to the global carbon cycle. It covers such topics as isolation and classification of microorganisms, metagenomics approaches, and field measurements.

Covers protein kinase inhibitors in research and medicine, and includes chapters on such topics as fragment based screening, broad kinome profiling of kinase inhibitors, and designing drug resistant kinase alleles.

Brings together a number of core protocols concentrating on DNA. Suitable for the researcher, this title contains step-by-step protocols.

Suitable for the researcher, this book brings together a number of core protocols concentrating on RNA.

Contains reviews and step-by-step protocols. In this volume, the authors have brought together a number of core protocols concentrating on Cell, Lipid and Carbohydrate, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes.