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In this century, students of biology are confronted with an entirely different scenario. All aspect of biology become more molecular-molecular biology. The tools have transformed our information management, taking access information to new heights. The advances made by the molecular biology tools have been very phenomenal in understanding and solving many of age old problems involved with many plant and animal genomes. These tools have been very dynamic when combined with traditional paths of research to know the structure and functions of millions of genes. The present book chapters contain first hands-on information on methods and protocols in a simplified manner which is very easy to learn and perform. Further, methods and protocols constitute a gold-standard reference for today's scientists who wish to develop and hone their molecular biology skills towards the discovery of new biological relationships. This book has been divided into 10 chapters with each chapter containing introduction, principle, protocol, applications and troubleshooting and it has been written keeping in mind the requirements of graduate/postgraduate students and research scholars.